FastQCFastQC Report
Mon 27 Feb 2023
SRR3127653.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127653.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2544542
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246710.9695654463553756No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC41550.16329068256684307No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG28140.11058964638823018TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG27930.1097643505196613TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC26340.1035156818004969TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81700.083.767741
CGTAGGG5950.075.0340352
AGGGATG52000.074.380085
TAGAGGG48950.074.116742
TAGGGAT23500.073.393634
TAGGGCA18000.071.538244
ATAGGGA30900.070.874113
ATAGGGT13600.069.802773
AGAGGGC43400.069.7358863
TAGGGTA9850.069.658934
AGTAGGG46800.069.488332
AGGGTAT16550.069.287045
ATAGGGC20800.068.686343
AAGGGTA19300.068.667624
AGATAGG15850.068.551751
AATAGGG47050.068.5198062
GAATAGG19400.068.372781
AGGGTAC11300.068.206475
AAGAGGG92500.068.079242
GTAGGGA21750.068.063063
Produced by FastQC (version 0.11.5)