FastQCFastQC Report
Mon 27 Feb 2023
SRR3127652.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127652.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1992521
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186390.9354481082006162No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29360.14735101913605927No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG25720.12908270477450426TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG25320.1270751977018059TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC22130.11106532879703651TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62450.085.3226551
CGTAGGG5400.079.362982
AGAGGGC32100.074.676353
TAGAGGG38600.072.471872
AGGGATG40800.071.299165
TAGGGCA14550.071.20824
ACGGGTA2000.070.641774
ATAGGGC15000.070.013853
ATAGAGG16500.069.098021
TAGGGAT17400.068.747164
AGGGCAT21050.068.316135
ATAGCGG4150.068.114151
CGAAGGG17700.067.848032
GTAGGGA15850.067.74483
AAGGGAT38950.067.70974
GAATAGG16250.067.551771
GGTAAGG13050.067.1485441
AAGGGTA16300.067.030234
AGTAGGG35650.066.975932
AAGAGGG74050.066.968972
Produced by FastQC (version 0.11.5)