FastQCFastQC Report
Mon 27 Feb 2023
SRR3127650.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127650.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1987315
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152770.7687256423868386No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG29010.14597585184029707No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG27810.13993755393583804No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC27110.1364152134915703No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24170.12162138362564565No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT65350.085.6026151
CGTAGGG7600.084.7160642
ACGGGTA2750.078.6111154
AGGGATT40250.075.777045
CGAAGGG18200.075.658032
TAAGGGA28800.075.389053
AGGGATG45200.074.964495
TAGGGCG3700.074.93924
AAGGGAT55500.074.5158164
TAGGGCA16550.074.398144
ATAGGGA31300.073.421513
TAACGGG6150.073.359252
GCGTAGG3850.073.323181
AGGGCAT27050.073.316895
ATAGCGG3600.073.187391
AAGGGCA41350.072.965624
GTAGGGA22950.072.899763
TAAGCGG5100.072.879891
GCGGGAT10800.071.7990954
TAGGGAT27750.071.6367954
Produced by FastQC (version 0.11.5)