FastQCFastQC Report
Mon 27 Feb 2023
SRR3127649.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127649.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1546572
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114710.7417048802124957No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC28150.18201545094570443No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG27410.17723067532581735No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG25550.1652040771461012No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC16780.10849802013743945No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50150.084.682791
CGAAGGG13150.078.823422
AGGGATG35100.078.324285
CGTAGGG5900.078.258652
AGGGCAT19950.076.793135
TAGCGGG5250.076.2812652
AGGGATT29650.075.444915
ATAGCGG2500.075.415631
AAGGGCA32800.075.4125754
AAGGGAT44050.074.97744
ATAGGGA22650.074.468593
ACGGGTA1750.072.691574
GAGGGAT35400.072.269474
TAGGGAT19350.072.072414
AGGGATC22000.070.9190755
TAAGGGA20500.070.787273
AGAGGGA47750.070.746363
CGAGGGA10800.070.6723563
GTAGGGA15300.070.518393
ATAGGGC12550.070.203093
Produced by FastQC (version 0.11.5)