FastQCFastQC Report
Mon 27 Feb 2023
SRR3127647.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127647.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3016512
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT299110.9915757006768081No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC72590.24064217215114675No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT40340.1337306133706745No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG40210.13329965204845862TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC39220.13001771582543015No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG36620.12139848938111301TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT92450.083.411691
CGTAGGG8400.078.328722
CTACGAC14250.072.886935
AGTAGGG50000.072.657722
TAGGGCA19750.071.626164
ATAGGGC21400.071.1546863
GTAGGGT12100.071.078453
TACGACC14600.070.817756
GGATAGG18050.070.639391
AGGGATG47800.070.397535
GAATAGG20450.069.251321
TAGCGGG17800.068.9116742
ATAGGGA28350.068.796653
GTAGGGA26000.068.688263
CGAAGGG28500.068.434562
AATGCGG7000.067.885681
TAGGGTA11350.066.665684
AAGGGCA48850.066.094364
AGGGCAT28850.065.975325
AGAGGGC46100.065.959243
Produced by FastQC (version 0.11.5)