FastQCFastQC Report
Mon 27 Feb 2023
SRR3127645.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127645.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2257297
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218640.9685920815913901No Hit
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG43910.19452469037082848RNA PCR Primer, Index 35 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC43420.19235395253703877TruSeq Adapter, Index 10 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG43020.19058192165231247RNA PCR Primer, Index 10 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36440.16143201359856502No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33270.14738866883710916No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT63350.085.31031
TAGGGCA13750.076.906214
CGTAGGG6500.076.644982
ATAGCGG5150.075.805181
ACGGGAT5150.072.094384
ATAGGGC14050.071.586093
AGTAGGG39200.070.618812
ATAGGGA17250.069.2047353
TATAGGG17000.068.8401262
AGTAAGG13500.068.288941
TAGCGGG12750.068.195042
GAATAGG11550.068.00841
GTAGGGA14450.067.652953
AGGGCAT21800.067.2635355
GGTAAGG13600.067.0951161
GAGGGTA10250.066.943834
AGATAGG11200.066.7739641
AGTACGG3100.066.7604141
TAGACGG2400.066.633971
GTAGGGC14700.066.502383
Produced by FastQC (version 0.11.5)