FastQCFastQC Report
Mon 27 Feb 2023
SRR3127644.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127644.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2695320
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292861.086550020034727No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC45040.1671044625499013No Hit
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG43680.16205867948889185RNA PCR Primer, Index 35 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG42350.1571242004659929RNA PCR Primer, Index 10 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC41020.15218972144309395TruSeq Adapter, Index 10 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT40000.14840538414733687No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85800.085.096091
CGTAGGG7200.078.981032
TAGGGCA17650.072.427284
AGTAGGG47750.071.94722
ATAGCGG6250.071.5070341
TAGAGGG48550.069.890462
TATAGGG20600.069.811042
GGTAAGG14900.068.51391
GTAGGGC15600.068.0866243
AGTAAGG16000.067.919921
AAGGGAC24450.067.8537454
ATAGGGA22800.067.817073
GAATAGG15350.067.7312551
GTATAGG9800.067.6859051
ATAGGGC17450.067.601493
TAAGGGA20700.067.204653
CTACGAC9950.067.072315
AGGGCAT27200.067.0410465
ATAGAGG20000.067.037861
ATAAGGG39750.066.918972
Produced by FastQC (version 0.11.5)