FastQCFastQC Report
Mon 27 Feb 2023
SRR3127643.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127643.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2110857
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214711.0171698035442478No Hit
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG38920.18438008827694155RNA PCR Primer, Index 10 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG38780.18371685054932665RNA PCR Primer, Index 35 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC38420.18201138210688833TruSeq Adapter, Index 10 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32690.1548660093980786No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30340.14373309039882853No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT63750.086.039531
CGTAGGG4600.076.800432
TAGGGCA13150.075.581754
AGTAGGG37400.073.931022
TAGGGTA7350.073.7005544
TAGAGGG33550.071.60412
CTACGAC7300.070.814265
ACGGGTA2000.070.65644
AGGGTAT11150.069.965535
AGGGCAT21500.069.945935
TAGCGGG11200.069.8152542
ACGGGAT4800.069.675064
ATAGGGC13400.069.601823
GTAGGGC12550.069.436563
GAGGGTA10250.069.3926244
AGAGGGC31150.068.652763
TATAGGG14000.068.3011862
AGGGTAC7500.067.672685
TAGACGG2650.067.571411
GCATAGG6850.067.415651
Produced by FastQC (version 0.11.5)