FastQCFastQC Report
Mon 27 Feb 2023
SRR3127642.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127642.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2198093
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186410.8480532898289563No Hit
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG37960.17269514984124876Illumina PCR Primer Index 7 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG35380.16095770288154324RNA PCR Primer, Index 16 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC31710.14426141205126444RNA PCR Primer, Index 16 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT59550.084.657331
CGTAGGG6250.077.444852
TAGACGG2900.076.216921
AGTAGGG35250.070.923122
CGAAGGG19700.070.8476642
ATAGCGG4200.070.54121
GAGGGAT31200.070.187014
GCGTAGG3150.070.167971
TAGCGGG11500.069.876892
TAAGGGA21200.069.823263
TAGGGCA16550.069.565444
TAGGGCG3650.069.522654
GTACGGG4450.068.64182
GGTAAGG11650.068.623771
AAGGGAT40350.068.479294
AGGGATG31300.068.1627045
AAGAGGG79150.068.1001052
AGAGGGC33550.067.651913
AAGGGTA16050.067.633584
TATAGGG17300.067.366022
Produced by FastQC (version 0.11.5)