FastQCFastQC Report
Mon 27 Feb 2023
SRR3127641.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127641.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2632113
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240980.9155382006775545No Hit
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG33490.12723617868989667RNA PCR Primer, Index 16 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG32280.1226391116186881Illumina PCR Primer Index 7 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC29030.11029161741916095RNA PCR Primer, Index 16 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78250.084.089521
CGTAGGG7850.076.630592
AAGGGTA21150.071.549634
ATAGGGA26150.071.5275343
AGTAAGG17050.071.171131
AGTAGGG43600.070.4941562
TAGGGCA19250.070.311064
ATAGGGC18250.069.270973
TAGCGGG12350.069.257422
AGGGATG39600.069.070045
ATAGCGG4900.068.150781
CGAAGGG25350.068.037762
TAGGGCG4300.067.7618264
AAGGGAT50100.067.726924
AGGGCAT27050.067.236575
TAAGGGA27250.067.088023
GAGGGAT35400.067.0424654
GTAGGGC13150.066.1161963
AGAGGGC37950.065.881233
AAGGGCA44650.065.6787644
Produced by FastQC (version 0.11.5)