FastQCFastQC Report
Mon 27 Feb 2023
SRR3127640.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127640.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2019639
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182830.9052607916563307No Hit
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG31480.15586944003359016Illumina PCR Primer Index 7 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG30330.1501753531200378RNA PCR Primer, Index 16 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC26830.13284552338313926RNA PCR Primer, Index 16 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT56100.084.924361
CGTAGGG6750.076.76262
TAGCGGG10150.073.324962
AGTAGGG34200.072.0338062
ATAGGGA20200.071.356053
CGAAGGG18900.070.78112
ATAGCGG4700.070.184981
TAGGGCA11800.068.660544
AGGGATG30400.068.327825
ATAAGGG31100.067.854462
GAGGGAT29400.067.45214
TAAGCGG5700.066.965971
AAGAGGG71750.066.635382
CGAGGGA13450.066.541423
AAGGGCA36100.066.415794
AAGGGAT38950.066.393524
TAGAGGG36450.066.2949752
GAATAGG12950.066.228611
GGTAAGG13000.065.9738851
AAGGGTA15000.065.9460454
Produced by FastQC (version 0.11.5)