FastQCFastQC Report
Mon 27 Feb 2023
SRR3127639.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127639.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1234835
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127631.0335793851000337No Hit
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG24990.202375216121992No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC23890.19346714338352897No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG21740.17605591030380577No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT17140.13880396976114218No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT38650.083.686041
ATAGGGC7900.079.115713
GAATAGG7500.077.100631
CGTAGGG3400.076.018972
TAGGGCA6800.073.9457244
AGGGATG17150.070.969845
GTAGGGA7750.069.732363
AGGGCGA7200.069.184945
TATAGGG9450.069.122782
TAGCGGG7300.068.880942
ATAGAGG7950.068.596981
TAAGAGG8300.068.536411
TAGAGGG19300.068.420652
GAGGGAT12900.067.758134
GGTAAGG7150.067.724411
AAGGGAT20150.067.6333854
ATAGGGA8200.067.6248553
AAGGGTA7400.067.315084
AGGGATA10000.067.200775
AAGGGAC11050.067.194394
Produced by FastQC (version 0.11.5)