FastQCFastQC Report
Mon 27 Feb 2023
SRR3127638.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127638.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1476089
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170971.158263492241999No Hit
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG23360.15825604011682223No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT20890.14152263176542879No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG20670.140032206730082No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC20280.13739008962196725No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT52350.085.283951
TAGGGCA8200.075.081634
AGGGCGA8300.074.177035
TAGCGGG8600.073.77542
ATAGGGC9550.072.8340763
AGGGATG22400.072.5946045
ATAGGGA11350.072.4633563
ACGGGTA1700.071.878784
GGTAAGG7700.069.651741
AGTAGGG23700.069.604132
CGAAGGG14250.068.60012
GAATAGG9900.067.954571
TAGGGCG2850.067.610674
AGGGCAT13800.067.431435
GTAGGGT7600.067.404543
ATGTAGG4200.067.207821
CGTAGGG4100.066.48452
TATAGGG12100.066.418182
TACGGGT850.066.349643
AGATAGG8300.066.317111
Produced by FastQC (version 0.11.5)