FastQCFastQC Report
Mon 27 Feb 2023
SRR3127637.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127637.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1114151
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126771.1378170463429105No Hit
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG21360.1917154856029389No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC21050.1889330979373532No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG19010.17062319200898263No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT16150.1449534219329337No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT37850.085.5470351
CGTAGGG2850.081.008112
TAGGGCA5600.074.882324
GAATAGG7250.074.7607961
ATAGGGC6600.074.244913
AGTAGGG17800.073.057762
GTAGGGA7450.070.201113
AGGGCAT10950.069.955025
AGGGATG15900.069.752585
GAGGGAT10900.069.594784
TAGAGGG19550.068.446132
AGTAAGG7100.067.710491
TAGCGGG6150.067.419392
AGGGCGA5100.067.266385
GGTAAGG5750.067.214051
AAGAGGG39500.067.03732
GGATAGG6550.066.9199751
AGGGACT14050.066.2268755
AAGGGTA8400.066.188124
ATAGGGA8700.066.072063
Produced by FastQC (version 0.11.5)