FastQCFastQC Report
Mon 27 Feb 2023
SRR3127635.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127635.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1393746
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140071.004989431359803No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30000.21524725452126856No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG26900.1930050382207375No Hit
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG26850.19264629279653536No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC24630.1767179959619615No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50100.085.148821
ATAGCGG2700.080.131481
AGGGATG31050.076.892495
AAGGGAT30350.075.414014
TAGCGGG7600.074.826182
CGAAGGG13950.074.792932
GGATGGC25850.074.3608867
AGGGCAT14350.074.0181665
TAGGGCA7650.073.7227554
TAAGGGA14000.073.518713
AGTAGGG21400.072.034712
AAGGGCA26300.072.016354
GGTAAGG8000.071.726381
CGAGGGA7900.071.389763
CGTAGGG4100.071.070542
AGGGTAC6300.070.124385
AGAGGGA33950.070.047483
AACGAGG5900.069.354771
TAGAGGG24100.069.22982
CGGGTAT1700.069.115095
Produced by FastQC (version 0.11.5)