FastQCFastQC Report
Mon 27 Feb 2023
SRR3127634.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127634.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1085317
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103930.9576004061486183No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG25780.23753428721746736No Hit
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG24410.22491124712871907No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC23370.21532879333872038No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC20820.19183335375747362No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT35450.085.4686661
AGGGATG26050.080.4594965
TAGCGGG5950.078.384482
AAGGGAT24650.076.828274
CGTAGGG2950.076.653352
GGTAAGG6450.075.9776461
CGAAGGG11050.075.461062
CGCTACG3900.074.892623
ACGGGTA1650.074.233744
TAAGGGA11350.073.881553
AGGGTAT7300.073.3892065
GTTACGG900.073.298951
ATAGCGG2650.072.90381
AAGGGCA20500.071.698924
GAGGGAT20200.071.3644564
AAGCGGG11200.070.66482
TAGAGGG17100.070.251562
GGATGGC20350.069.741737
AGAGGGA25850.069.434663
AGGGAAT16000.069.317395
Produced by FastQC (version 0.11.5)