FastQCFastQC Report
Mon 27 Feb 2023
SRR3127633.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127633.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2531963
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227580.8988283004135527No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC34520.13633690539711676No Hit
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG32640.1289118363893943Illumina PCR Primer Index 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG32590.12871436114982723RNA PCR Primer, Index 10 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC29690.1172607972549362TruSeq Adapter, Index 10 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71000.083.791131
CGTAGGG7550.078.429712
ATAGGGC14900.074.434473
AGGGATG39950.069.873025
TAGGGCA16200.068.458634
AAGAGGG90450.068.116312
GAATAGG14950.067.948271
AGGGTAC12600.067.8798145
GGTAAGG15850.067.6505661
GTAGGGA19900.067.540053
AGTAGGG40100.067.3876652
AGGGCAT25300.067.054285
ATAGGGA25250.066.816153
AGAGGGC40900.066.527883
TAGAGGG50350.066.17652
TAGCGGG12550.065.906152
GAGGGAT35900.065.580474
AGATAGG15500.065.53721
AGGGAAT23100.065.3029565
TAAGGGA22000.064.938063
Produced by FastQC (version 0.11.5)