FastQCFastQC Report
Mon 27 Feb 2023
SRR3127632.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127632.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3042147
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300270.9870331709808895No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC43160.14187348606099573No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG33800.11110574209596052RNA PCR Primer, Index 10 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC31680.10413697957396535TruSeq Adapter, Index 10 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG31270.10278924719942856Illumina PCR Primer Index 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT93050.082.641581
CGTAGGG9400.075.497432
AGGGTAC14050.071.250245
TAGCGGG14700.069.058872
TAGGGTA12050.069.0361254
TAGGGCA18050.068.480784
ATAGGGC18400.068.199883
ATAGGGA30350.067.827473
TAGAGGG62100.067.432492
AGGGCAT27450.067.287325
AAGAGGG113200.066.428832
AGTAGGG51650.066.425612
AAGGGTA23750.066.095674
AGAGGGC48600.065.8568653
CGAAGGG34450.065.483982
AGGGATG46450.065.3626865
ATAGAGG25700.065.1499561
AAGGGCA52750.065.0415044
ATGCGGG14600.065.025182
AAGGGAT49650.064.464124
Produced by FastQC (version 0.11.5)