FastQCFastQC Report
Mon 27 Feb 2023
SRR3127631.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127631.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2357560
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222500.9437723748282122No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31430.13331580108247512No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG28920.12266920035969393RNA PCR Primer, Index 10 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG28380.12037869661853781Illumina PCR Primer Index 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC24310.1031150850879723TruSeq Adapter, Index 10 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69850.083.9936451
CGTAGGG8200.080.414812
ATAGCGG4700.077.203381
TAGCGGG13000.071.374772
ATAGGGC13750.071.249613
AGGGTAC10950.070.814715
TAGGGCA14850.070.09514
CGAAGGG27350.069.229412
AGGGATG37100.068.402745
AGAGGGC37600.068.395363
TAGGGTA8250.067.367424
TAGAGGG46600.067.314742
ATAGGGA23650.067.3143163
AAGAGGG87800.066.7877352
GTAGGGC12050.066.057413
AGTAGGG39200.065.844022
GTAAGGG33150.065.4996952
GAGGGAT35750.065.3472754
TAGGGCG3750.065.312144
TAAGGGA18300.065.116533
Produced by FastQC (version 0.11.5)