FastQCFastQC Report
Mon 27 Feb 2023
SRR3127630.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127630.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences787203
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82531.0483953948346234No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG29960.38058798048279796No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG23360.2967468365847183No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC22430.2849328572172616No Hit
AGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCT9850.12512655566607342No Hit
AGAGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTT8640.10975567928475884No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT27650.088.2803341
CGTAGGG2300.085.813272
TCGTAGG353.8449798E-880.625971
ACCGATG850.077.400219
AACGAGG3100.077.374921
GGTAAGG4450.077.153321
AGGGATG14650.076.664325
ACGGGTC800.076.3635944
GGATGGC9200.075.5974047
AGTACGG507.094059E-1175.25091
CGGGTAT750.075.1887745
TAGGGTA2200.074.761564
CGGGAAT1700.074.635915
AGTAAGG5550.074.572971
TAGGGCG1450.074.5405964
GTACGGG1450.074.5405962
AAGGGCA12350.073.818934
GAGGGTA4400.073.6935354
ACGGGTA900.073.100194
TAGCGGG2900.072.920142
Produced by FastQC (version 0.11.5)