FastQCFastQC Report
Mon 27 Feb 2023
SRR3127629.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127629.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923366
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113081.224649813833301No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG27060.2930582239328717No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG22140.23977491049053137No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC20340.22028101532869954No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA9240.10006866183073666No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG2500.086.4678962
CGTTTTT37250.085.257431
TAGCGGG3900.078.3223652
AGTAAGG6250.076.7847441
AGGGCAT8800.076.364315
ATAGCGG1050.076.175331
AACGAGG4350.075.711791
AGGGATG16300.075.247145
AGGGTAT6000.075.189475
TAACGGG2900.074.541292
TAGGGTA3200.073.4272164
AGTAGGG13250.073.416142
CGGGTAT1350.073.100885
TAGGGCG2250.073.100884
GTAAGGG15650.072.967422
AGGGCAA10450.072.851045
AAGGGCA14750.072.640684
GGTAAGG5800.072.196611
AGGGATT12650.072.0689545
AAGGGAT20200.071.885984
Produced by FastQC (version 0.11.5)