FastQCFastQC Report
Mon 27 Feb 2023
SRR3127628.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127628.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences726771
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83211.1449273567602452No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG27780.3822386969210384No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG21630.29761781909294677No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC19470.2678973156606414No Hit
AGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCT9730.13387986036867183No Hit
AGAGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTT8460.11640530510986266No Hit
AAAGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTT7490.10305859754998478No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT500.093.992025
CGTAGGG2100.087.440732
CGTTTTT26900.086.6883851
TAGGGTA1700.083.088484
ACGGGTA353.8153303E-880.714524
AGGGTAC2750.080.320455
CGATAGT301.4563357E-678.3266759
GGGCGAT2900.077.78656
AGTAAGG4250.077.592031
AACGAGG3700.076.393711
AGGGATG13450.075.4731455
GTACGGG1500.075.333562
TAAGGGA7150.075.0701453
TAACGGG2700.074.9847952
GCGTAGG1200.074.5899661
TAAACGG1150.073.736531
AAGGGTA4750.073.351094
TAGCGGG2450.073.027422
TAGAGGG13150.072.3259352
CTAGCGG850.072.049751
Produced by FastQC (version 0.11.5)