FastQCFastQC Report
Mon 27 Feb 2023
SRR3127627.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127627.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1885624
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148910.7897120528801076No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG33170.1759099375060988No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32990.1749553463468857No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG32500.172356737080139No Hit
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC29270.1552271290564821TruSeq Adapter, Index 14 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT49800.083.677331
TAGGGCG2600.077.7215354
AGGGATG43750.077.66375
AAGGGAT46500.075.494264
CGAGGGA10700.075.105223
ATAGCGG3250.073.8057561
CGGGATG8400.073.2908555
TAAGGGA19800.072.867013
TAGCGGG9400.072.495232
AGTACGG2600.072.358581
GAGGGAT35300.071.223834
TAAACGG2050.071.123191
CGAAGGG18200.070.753592
GGATGGC32100.070.7131047
AGAGGGC31500.070.568093
AGGGATT27000.069.7972645
AGGGCGA8150.069.7727055
TAGAGGG32250.069.51172
GAATAGG13100.069.2930151
TAGGGCA13500.069.2732544
Produced by FastQC (version 0.11.5)