FastQCFastQC Report
Mon 27 Feb 2023
SRR3127626.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127626.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2282342
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198410.8693263323375725No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC43360.18998029217356557No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG33610.1472610152203307No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG32240.14125840912536333No Hit
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC29660.12995423122389194TruSeq Adapter, Index 14 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT72750.084.007821
TAGGGCG3950.080.9057544
AGGGATG51100.079.094295
AAGGGAT58900.074.604314
TAGGGCA20100.074.353034
AAGGGTA19450.074.179924
TAGCGGG10850.073.202272
TAAGGGA26900.072.504243
GAGGGAT45750.072.215734
CGAAGGG20850.071.903862
CGAGGGA15150.071.658373
ACGGGTA3500.071.1664664
TAGGGTC5800.070.4950944
GGATGGC40150.070.465837
AGTAGGG36500.070.301952
GTAGGGC12050.069.812563
ATAGGGC19150.069.6974953
GAATAGG16150.069.62561
TAGAGGG43150.069.4876252
AGAGGGC37750.069.094523
Produced by FastQC (version 0.11.5)