FastQCFastQC Report
Mon 27 Feb 2023
SRR3127625.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127625.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1757575
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148610.8455400196293188No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29920.17023455613558452No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG28330.1611880005120692No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG27960.15908282719087377No Hit
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC26580.15123109966857745TruSeq Adapter, Index 14 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT54900.085.0051961
ATAGCGG3600.077.255981
TAGCGGG9250.076.9230962
AGGGATG41700.076.408195
TAGGGCA15600.076.119774
CGGGATG8600.075.956085
TAGAGGG31500.074.646862
TAAGCGG3250.073.972361
TAAGGGA19850.073.828053
AAGGGAT45150.073.0574
AAGGGTA15100.072.711074
CGAGGGA10000.072.567513
TACGCGG650.072.521911
TAGGGCG3000.072.253374
CGAAGGG15450.071.6738742
AGGGCAT23950.071.423665
GGATGGC31300.071.31747
AGGGTAT12300.071.064715
GCGGGAT8200.070.682644
AGAGGGC30050.070.565033
Produced by FastQC (version 0.11.5)