FastQCFastQC Report
Mon 27 Feb 2023
SRR3127624.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127624.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2318746
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204370.8813815743509639No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG42550.1835043596840706No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG40770.17582779657625286No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC39500.17035069817910198No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27390.1181241929905216No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23530.10147726400390555No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69100.083.1710361
TAGGGCA14700.079.2822654
AGGGATG34150.076.788215
GTAGGGT10950.076.391943
ACGGGTA2850.075.849834
TAGACGG3600.073.158451
GGTAAGG14150.073.121511
ATAGGGC16050.072.028063
AGGGTAC12750.071.87465
AGTAAGG13850.071.649251
AGGGTAT17800.071.548465
AAGGGTA21300.071.483714
GAGGGTA15900.071.2297744
CGAAGGG23550.071.039642
AGTAGGG40050.070.520222
AGGGCAT25850.070.174255
ATAGGGA22200.070.067513
CGTAGGG5900.069.296122
GTAGGGA16300.068.616893
AGATAGG17300.068.506751
Produced by FastQC (version 0.11.5)