FastQCFastQC Report
Mon 27 Feb 2023
SRR3127623.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127623.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2761447
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271980.9849184141502626No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG42810.15502741859612007No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC40390.14626389715247115No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG37880.13717445962207495No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33190.12019061021268922No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29960.10849384398831483No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85150.085.805351
TAGGGCA19100.079.9689254
CGTAGGG8200.076.2270362
ACGGGTA5250.076.0905764
ATAGGGC19800.074.768143
AGGGATG44600.072.91935
GTAGGGA20200.071.891643
GTAGGGT14250.071.567543
GAATAGG15250.070.955421
AGTAGGG47600.070.693152
CGAAGGG28600.070.662
GGTAAGG18250.070.118661
AGTAAGG17000.070.0163041
AGGGTAC15900.069.756745
GCATAGG8450.069.595461
TAGCGGG12500.069.555742
TAGAGGG48950.069.319562
AAGGGTA26300.069.155464
AGGGTAT23450.069.142885
TAGGGCG4700.068.995774
Produced by FastQC (version 0.11.5)