FastQCFastQC Report
Mon 27 Feb 2023
SRR3127622.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127622.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2125357
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195520.9199395677996683No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG37000.17408840020758867No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG33770.1588909533786559No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC33010.1553150835365541No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26140.12299110220071263No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC21820.10266510520350228No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60400.084.181181
CGTAGGG5800.077.145582
TAGGGCA15000.076.929024
AGGGATG35250.074.7924355
ACGGGTA3750.074.1030654
CGAAGGG22400.074.013342
TAGGGTA9950.073.844294
GGTAAGG14600.073.2661741
GAGGGTA15650.072.229114
TATAGGG18600.071.6619952
GTAGGGT11700.071.2529453
AGGGTAT16750.070.70335
AGTAAGG12750.070.591711
AGTAGGG39600.070.292292
AATGCGG4900.070.203261
AGGGTAC13150.070.046155
ATGCGGG9900.069.935482
ATAGGGC15300.069.571663
AGGGCAT22300.069.544545
TAGACGG3050.069.525351
Produced by FastQC (version 0.11.5)