FastQCFastQC Report
Mon 27 Feb 2023
SRR3127621.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127621.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1906534
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296571.555545298431604No Hit
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG22240.11665147330181366RNA PCR Primer, Index 34 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG19710.10338131918969187TruSeq Adapter, Index 18 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC19640.10301416077552249RNA PCR Primer, Index 34 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT82250.087.376281
TAGGGCA10650.077.66744
ATAGGGC11450.076.345453
GAATAGG11650.073.4770361
AGGGCAT17300.072.26415
AAGGGTA11550.071.208494
AGTAGGG31150.069.251682
ATAGGGA13650.068.8609543
ATAAGGG27950.068.772872
AGATAGG9850.068.281951
TAGAGGG36600.067.414592
CGTAGGG5100.067.271072
TAGGGTA5800.067.255194
AAGAGGG74200.067.202772
GTAGGGC10150.067.1394353
TAGCGGG10700.066.762952
GGATAGG12050.066.744451
TATAGGG16850.066.382372
GGTAAGG12800.066.140681
TAAGGGA16000.065.796643
Produced by FastQC (version 0.11.5)