FastQCFastQC Report
Mon 27 Feb 2023
SRR3127620.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127620.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2287559
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT375331.6407445665882279No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT108750.087.207011
TAGGGCA15800.074.065254
ATAGGGC15450.070.8760763
GAATAGG13850.070.648611
AGTAGGG38450.070.52642
TAGAGGG44050.069.028842
AGGGCAT22300.067.650715
CGTAGGG6850.067.236912
TAGCGGG13850.067.187362
TAGGGTA7000.067.138894
AGAGGGC39900.067.138893
AAGAGGG90250.065.978382
TAGGGTC5500.065.796114
ATAGGGA17750.065.663733
AAGGGTA18000.065.273924
AGACGGG15150.065.144672
TGTAGGG19500.065.073082
GGTAAGG15700.065.020481
GAGGGAT24850.064.4911654
ATAAGGG33500.064.252922
Produced by FastQC (version 0.11.5)