FastQCFastQC Report
Mon 27 Feb 2023
SRR3127619.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127619.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1738403
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288851.6615824984195264No Hit
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG20320.1168888917011763RNA PCR Primer, Index 34 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC17840.10262292460378865RNA PCR Primer, Index 34 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT79050.087.4118041
TAGGGCA11350.075.1311954
ATAGGGC11050.073.3337253
TAGAGGG31700.070.594672
CGTAGGG4950.070.431042
GAATAGG11250.070.3873751
AGGGTAG5950.069.504865
TAGCGGG8950.068.958192
ATAGGGA14500.068.557043
ATAAGGG25300.068.52752
AGTAGGG26950.068.003062
AGAGGGC28850.067.933673
TAAGAGG14750.067.1066061
AATAGGG30450.066.994352
AAGAGGG68650.066.980262
AGACGGG12950.066.939992
TAGGGTA5000.066.899974
GTACGGG3600.066.742932
GGTAAGG9350.066.0385741
TATAGGG15300.065.588212
Produced by FastQC (version 0.11.5)