FastQCFastQC Report
Mon 27 Feb 2023
SRR3127618.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127618.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2526949
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300521.1892602502068699No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG26890.10641291138048295No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26840.10621504430837346No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT93100.085.327451
CGTAGGG7400.076.216472
ATAGGGC16450.071.713113
TAGGGCA17250.071.384334
ATAGGGA19350.071.1667943
GGTAAGG15350.070.47391
AGTAGGG45100.070.135492
AGGGATG32850.070.10545
AGGGCAT28050.069.53545
ATGCGGG14300.069.021212
GAATAGG16500.068.697661
AGATAGG16000.067.9048541
ATAAGGG36050.067.794962
AAGGGTA21150.067.7766654
AGAGGGC43950.067.691723
GATAGGG34850.067.566942
TAGGGTA8300.067.384444
AAGAGGG92250.067.201312
TATAGGG20850.066.949872
AAGGGCA50600.066.7834854
Produced by FastQC (version 0.11.5)