FastQCFastQC Report
Mon 27 Feb 2023
SRR3127617.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127617.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2992456
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT382981.279818316459791No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32280.10787126026247336No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT119350.084.802381
ATAGGGC21650.074.460393
CGTAGGG8000.071.086062
TAGGGCA21300.070.829554
GATAGGG46650.069.617022
ATAGGGA27850.069.359463
AGAGGGC49950.069.251853
ATAAGGG43800.068.8890152
TAGAGGG56950.068.3322452
AGGGCAT31600.068.118875
AGTAGGG52300.068.0273062
AAGGGTA26000.067.6063164
AGGGATG39700.067.598135
GAATAGG19800.067.2529451
AAGAGGG112200.067.1474842
GGATAGG18700.066.679811
GGTAAGG18500.066.38331
AGATAGG22200.066.128961
CGAAGGG29700.065.6720662
TAAGGGA25700.065.469493
Produced by FastQC (version 0.11.5)