FastQCFastQC Report
Mon 27 Feb 2023
SRR3127616.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127616.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2324155
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294811.2684610105608274No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24990.10752294920089236No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG23860.10266096710417334No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT89050.084.941011
CGTAGGG7050.079.512862
CGGGTAT2350.073.991965
ATAGGGC16350.073.468643
ATAGCGG4900.071.1724551
GGTAAGG15550.070.615791
TAGGGCA16700.070.236444
GAATAGG15900.069.0613561
AAGGGTA21500.068.79724
AGATAGG16250.068.733951
AGGGCAT25050.068.663195
AGTAAGG16550.068.057531
AAGGGCA49300.067.554154
TAGAGGG42750.067.436482
AGGGTAG11650.067.3660665
ATAAGGG32950.067.1926962
AGAGGGC38350.066.5753563
ATAGGGA20950.066.556013
TAGCGGG11550.066.479122
GAGGGAT25050.066.381434
Produced by FastQC (version 0.11.5)