FastQCFastQC Report
Mon 27 Feb 2023
SRR3127615.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127615.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1977911
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178130.9005966395859065No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39020.19727884621704414No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG31980.16168573813483011No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG30110.15223131880049204No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC27300.13802441060290377No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT57400.083.5862961
CGTAGGG6950.077.089172
AGGGATG35500.074.1310355
TAGGGCA13150.073.260274
AGGGTAC9950.072.2617955
ATAGCGG3650.072.167551
ATAGGGC14300.069.343983
TAAACGG1800.067.943461
TAGGGTA7850.067.64714
TACGGGT1600.067.556983
AGTAGGG33600.067.4188162
CGAAGGG18100.067.250342
AAGAGGG73600.067.23942
GAGGGAT26400.066.752534
AGTACGG2750.066.708121
TAAGAGG14250.066.678111
ATAAGGG29850.066.599252
AAGGGAT38950.066.237714
GAATAGG13300.066.1358261
AGGGCTA9950.066.12195
Produced by FastQC (version 0.11.5)