FastQCFastQC Report
Mon 27 Feb 2023
SRR3127614.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127614.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2368989
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239171.0095867899766524No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC49230.20781016712192418No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG29840.12596090568592763No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG29550.12473675479286735No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC27640.11667424373857371No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74000.084.315141
ATAGCGG4000.081.1676251
CGTAGGG6800.076.714212
TAGGGCA16650.076.492144
AGGGATG44900.074.209875
TAGGGTA9100.073.8510064
AGGGTAC12400.071.2521445
AAGGGAT48250.069.544484
GGATGGC36900.069.4115757
AGTACGG2850.069.3422851
GAGGGAT36350.069.168944
AGGGTAA14000.069.15145
ATAGGGC15450.069.049283
AGTAGGG39550.068.682032
GGTAAGG14000.068.5639651
GAGGGTA16300.068.043434
AAGGGTA18600.067.7148064
ATAGGGA21700.067.353853
GTAGGGC11450.067.313193
TAGAGGG45150.067.0331952
Produced by FastQC (version 0.11.5)