FastQCFastQC Report
Mon 27 Feb 2023
SRR3127613.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127613.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1815998
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174670.9618402663439057No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35720.1966962518681188No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG28700.15803982162975952No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG24920.13722482073218142No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC23690.13045168551947744No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT57400.084.742021
ACGGGTA3100.079.017074
CGTAGGG5800.077.9690862
TAGGGCA12600.077.01514
AGGGATG33600.075.807065
AGGGTAC10600.074.48235
TAGGGCG3650.072.272724
ATAGCGG2550.072.086751
CGAAGGG18200.071.953622
AAGGGAT32950.069.908934
AGTAGGG30500.069.1922452
AGAGGGC26700.068.983423
AGTACGG2050.068.976061
ATAGAGG13750.068.558031
TAGAGGG32750.068.465992
GAGGGTA12800.068.083374
AGGGAAT22300.068.068675
AGGGCAC18550.067.8954245
TAGGGTA8050.067.879924
GTAGGGC9800.067.2947543
Produced by FastQC (version 0.11.5)