FastQCFastQC Report
Mon 27 Feb 2023
SRR3127612.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127612.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2111602
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200380.948947765724791No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG53460.25317270962993976No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC48650.23039379580053437TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG48630.23029908098211688No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26200.1240764121269065No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62850.083.955341
CGTAGGG6950.077.089062
TAGGGCA12400.076.5582664
GTACGGG4700.074.995662
AGTAGGG32050.072.732142
TAGGGTA7250.072.6009754
ACGGGTA2350.071.994124
GAATAGG11600.071.353521
GGTAAGG14050.070.961091
GTAGGGT7650.070.6495063
TAGACGG3200.070.542691
GGATAGG11250.069.8111341
TAGTAGG8550.069.30511
AGAGGGC29900.069.159873
AAGAGGG72250.068.625782
TGTAGGG14800.068.273072
ATAGGGC13500.067.8849643
AGTTAGG6750.067.581641
ATAGGGA17150.067.4133
TAAGGGA14650.067.368113
Produced by FastQC (version 0.11.5)