FastQCFastQC Report
Mon 27 Feb 2023
SRR3127611.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127611.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2528887
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275661.0900447509121602No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC51070.20194654802685924TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG50510.199732135125057No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG49610.1961732572471605No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32480.1284359483045308No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81550.083.525981
CGTAGGG8000.083.416962
TAGGGTA8950.075.612834
GAATAGG15000.073.3840261
GTAGGGT9650.073.049963
GTACGGG5350.072.908862
TAGGGCA16050.072.030454
ATAGCGG4650.071.826121
AGTTAGG8250.071.2743151
TGTAGGG17300.070.900682
AGTAGGG37900.070.803212
AGTACGG3000.070.561571
AGAGGGC37600.069.9932563
GGATAGG13100.069.663841
AGTAAGG16350.069.051081
TAGACGG3350.068.8063051
TAGAGGG46550.068.549792
ACGGGTA2950.068.501884
TAGTAGG10200.068.255631
GAGGGTA15600.068.083184
Produced by FastQC (version 0.11.5)