FastQCFastQC Report
Mon 27 Feb 2023
SRR3127610.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127610.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1975138
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199211.008587754374631No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG47030.23810994472284974No Hit
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG42080.21304840471906267No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC39920.20211245999013738TruSeq Adapter, Index 14 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24270.12287749007917421No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT59600.084.0745541
ATAGCGG2700.076.818621
CGTAGGG8000.074.213292
GAATAGG11800.073.9038851
TAGGGTA6650.073.690734
CGAAGGG18650.072.258412
TAGGGCA13250.071.83514
ATAGGGC13100.071.2188643
GTACGGG3850.070.98532
AGTAGGG29550.070.798932
GTAGGGT8550.070.541553
GGATAGG10850.068.2099151
AGAGGGC28900.067.989113
AGGGTAA11450.067.7210545
GGTAAGG12200.067.616981
GTAGGGA13250.067.5676653
GACCGAT1600.067.554318
TAAGAGG15250.067.3851551
AAGAGGG67850.067.363252
ACGGGTA2250.067.014474
Produced by FastQC (version 0.11.5)