FastQCFastQC Report
Mon 27 Feb 2023
SRR3127609.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127609.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2024792
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180040.8891777525790303No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG38030.18782176144512622No Hit
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG35880.17720338681701625No Hit
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC34480.1702890963615028TruSeq Adapter, Index 16 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT56000.083.8971941
ATAGCGG3500.079.278231
TAGCGGG9950.072.746172
TAGGGCA16800.072.1792144
ATAGGGC16050.071.7469563
AGAGGGC32350.070.756633
AGTAGGG32000.070.6492542
AGGGATG28700.070.254985
AAGGGTA15000.069.8739554
AGTAAGG12550.069.701031
CGGGTAT2500.069.560625
GGTAAGG12050.069.47091
TATAGGG15950.068.6606142
CGTAGGG6400.068.299172
GAATAGG12200.067.845771
TAGAGGG38300.067.741182
AGGGTAC11100.067.324935
ATAAGGG28350.067.3109052
GTAGGGC12050.067.089393
GTACGGG4000.066.977262
Produced by FastQC (version 0.11.5)