FastQCFastQC Report
Mon 27 Feb 2023
SRR3127608.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127608.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2418331
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236970.9798906766691574No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG36890.15254322092385203No Hit
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG35650.14741571769952086No Hit
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC34430.14237091614009825TruSeq Adapter, Index 16 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24500.1013095395129947No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75150.084.742731
ATAGGGC16850.073.9093553
ATAGCGG4450.072.929261
AAGGGTA19750.070.90924
TAGGGCA18400.070.237384
AGTAAGG17300.070.1433261
AGGGATG30200.069.870335
AGAGGGC37350.069.580573
GAATAGG15250.069.394561
GGTAAGG16300.068.386991
TAGCGGG11400.068.0193562
TATAGGG20300.067.8305052
AGTAGGG41700.067.731682
AGGGTAC14450.067.6470645
CGGGTAT2850.067.607125
CGAAGGG24900.067.001182
CGTAGGG7400.066.6823652
TGTAGGG19700.066.556642
AGGGCAT25650.066.507825
TAAGGGA20750.066.3594743
Produced by FastQC (version 0.11.5)