FastQCFastQC Report
Mon 27 Feb 2023
SRR3127607.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127607.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1867375
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175130.9378405515764108No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG33520.1795033134747975No Hit
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG32900.1761831447888078No Hit
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC29970.1604926701921146TruSeq Adapter, Index 16 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT53850.084.932481
ATAGCGG3350.077.411541
TAGGGCA14950.074.7050554
ATAGGGC14150.073.9331743
GTACGGG3950.071.580872
AGTAGGG29150.071.4539952
CGTAGGG4650.069.926052
CGAAGGG20500.069.881552
TAGGGTA6350.069.758484
AGAGGGC27700.069.410193
AGGGATG23850.069.3581855
AGATAGG11000.069.011421
TAGAGGG33050.068.8681642
GGTAAGG11900.068.546761
AGTAAGG11600.068.287161
AAGGGTA15400.067.9321064
GAATAGG11200.067.7790761
AGGGTAC10350.067.653365
TAAGGGA15150.067.497853
GAGGGAT22150.067.016184
Produced by FastQC (version 0.11.5)