FastQCFastQC Report
Mon 27 Feb 2023
SRR3127606.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127606.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436433
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108110.7526282116882583No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC47610.33144601941058166No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG45630.3176618749360395No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG40420.2813914745762594No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA15280.10637460988434547No Hit
AGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT15220.10595690853663206No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT43200.085.029011
TAAGGGA18850.080.785053
CGTAGGG2800.080.571342
CGAAGGG14750.079.023642
GGGCGAT6550.076.773196
CGAGGGA11450.076.349263
AGGGATG29800.076.338145
TAGGGCG2350.076.002564
ATAGCGG2600.075.975891
GGCGATG3100.075.801097
AGGGATT37250.075.707255
ACGGGTA1250.075.202544
AGGGCGA6650.074.9198155
AAGGGAT44700.074.340314
AGGGAAT23800.074.25465
ATAGGGA21100.073.952533
AGCGGGA11900.073.8570563
GAGGGAT38300.073.754444
TAGGGAT20850.073.7147144
GCGGGAT11250.073.5313644
Produced by FastQC (version 0.11.5)