FastQCFastQC Report
Mon 27 Feb 2023
SRR3127604.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127604.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1344874
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108290.8052055434189375No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC44500.3308860160877525No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG40150.2985409785600733No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG38020.28270306363272696No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA15080.11212946342928781No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT44000.085.696111
ATAGCGG2550.085.023981
ACGGGTA1250.082.9229054
AGGGATG28400.080.420035
CGAAGGG11900.079.977222
GTACGGG3500.079.422912
TACGGGT600.078.525493
AGGGATT35050.077.63135
TAGCGGG6650.077.226572
TAGGGCG1900.076.8723144
AGCGGGA13050.076.539793
AAGGGAT41250.075.955564
GCGGGAT9750.075.86774
GAGGGAT34600.075.711274
TAAGGGA17800.075.43743
AGGGCGA6250.075.191085
CGTAGGG2950.075.065032
CGGGATT6400.074.897365
TAGGGAT19350.074.751394
GGGCGAT5500.074.336636
Produced by FastQC (version 0.11.5)