FastQCFastQC Report
Mon 27 Feb 2023
SRR3127603.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127603.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2069754
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151870.7337586978935661No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC49090.23717794481856297No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG45340.2190598496246414No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG43530.2103148490110419No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC21630.10450517307853976No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT58000.084.633831
AGGGATG46700.077.5888065
TAGGGCA17900.076.9246754
CGTAGGG5750.076.828362
AGGGATT40300.076.733055
AAGGGAT59950.076.273694
CGAAGGG15450.076.045432
TAAGGGA29300.074.422023
GTAGGGC12700.074.377823
AAGGGCA43100.073.927084
AGTACGG2550.073.754971
TAGGGCG3700.073.667744
TAGCGGG9800.073.371432
AGGGCAT25150.073.250345
AGGGAAT28900.072.852045
GAGGGAT44450.072.6333544
ATAGGGA29550.072.520113
AGGGATC26750.072.0313645
CGAGGGA16100.071.222143
AGAGGGA64950.071.125523
Produced by FastQC (version 0.11.5)