FastQCFastQC Report
Mon 27 Feb 2023
SRR3127602.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127602.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2481436
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202670.8167448203379011No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC49530.19960216584268142No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG44520.17941224355574756No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG42060.1694986290196483No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26740.1077601840224773No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT79000.084.786991
CGTAGGG6650.084.1032262
ATAGGGA39250.076.275863
CGAGGGA20350.076.214373
AAGGGAT76300.075.5184864
AGGGATG57800.075.4568565
CGAAGGG19750.074.722242
TAGCGGG11650.074.6333852
GTAGGGC14750.074.560913
AGGGATT53150.074.4538965
AAGGGCA48950.073.834784
TAGGGCA22450.073.6908654
GTACGGG5450.073.300982
GAGGGAT56900.072.769774
AGAGGGC43750.072.297673
AGGGCAT33400.071.903995
AGGGAAT35150.071.8005145
AGGGATC35250.071.7301565
ATAGGGC21100.071.500643
TAGGGCG5200.071.4021154
Produced by FastQC (version 0.11.5)