FastQCFastQC Report
Mon 27 Feb 2023
SRR3127601.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127601.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1925740
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146650.7615254395712817No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC41800.21705941612055624No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG39140.20324654418561175No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG38510.1999750745168091No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC19340.10042892602324302No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT57150.085.766881
CGTAGGG5900.079.846942
TAGGGCA16800.078.235754
AGGGATG46700.077.6871955
TAGCGGG10500.077.1701662
AAGGGAT61450.077.123444
CGAAGGG15200.076.863192
AAGGGCA37250.074.490234
GTAGGGC12800.074.344993
GAGGGAT42250.074.260484
ATAGCGG4400.073.909271
AGGGATT40050.073.689595
ATAGGGA29100.073.011933
AGAGGGA58600.072.915523
AGGGATC26250.072.864195
AATGCGG3050.072.62741
ACGGGAT10200.072.050124
CGAGGGA15450.071.350613
AGGGCAT25200.071.051545
AGCGGGA17900.070.0065843
Produced by FastQC (version 0.11.5)