FastQCFastQC Report
Mon 27 Feb 2023
SRR3127600.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127600.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2592954
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289931.1181455590804927No Hit
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC33260.1282706904943165TruSeq Adapter, Index 16 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG31280.12063461210650092TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG30750.11859061132592404TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT88400.085.547011
TAGGGCG4350.074.5468444
ATAGGGC19150.074.113823
CGTAGGG6200.072.768032
TAGGGCA19500.072.061954
GAATAGG16550.070.473331
AGTAGGG43850.070.413642
AGGGATG34900.070.028125
ATAGCGG5400.068.802531
AAGAGGG92300.068.330072
AGAGGGC44650.067.994243
CGAAGGG22350.067.4976352
ATAGGGA23200.067.455493
AGGGCAT28650.067.259535
AATAGGG41450.067.120972
TAGAGGG50500.066.911162
ATAAGGG40500.066.8387762
AAGGGAT44050.065.82774
GGATAGG17000.065.5647661
AAGGGCA49550.065.255064
Produced by FastQC (version 0.11.5)