FastQCFastQC Report
Mon 27 Feb 2023
SRR3127599.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127599.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3113195
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT381681.2260073654236243No Hit
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC33440.10741376624336092TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG31370.10076464853631076TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT114950.084.6301351
CGTAGGG7650.075.562872
TAGGGCA24700.072.8729864
ATAGGGC23550.072.839463
TAGCGGG13600.070.149072
GGATAGG20550.069.360831
ATAGGGA29250.069.088753
ACGGGTA3750.068.928084
TAGGGAT21300.068.839824
AGTAGGG52850.068.382682
GAATAGG18350.067.934961
AGGGCAT37550.067.459585
TAAGGGA33100.067.441993
AAGAGGG117350.067.3208162
AGGGATG44450.067.137735
CGAAGGG29200.066.6319052
ATAAGGG48350.066.5823062
GTACGGG7000.066.466362
ACGGGAT9450.066.1431054
AGTAAGG22700.066.107131
Produced by FastQC (version 0.11.5)